Solution Brochure

CLEVER + Antibiotics — peptide synergy that boosts efficacy and cuts resistance

A 4-page infographic series explaining the synergy between yeast-derived peptides (CLEVER) and antibiotics: enhanced membrane permeability, biofilm disruption, and immune modulation — for lower antibiotic doses and stronger Vibrio kill.

As antimicrobial resistance becomes a major threat to shrimp aquaculture, antibiotic-only protocols are losing efficacy and leaving long-term environmental damage. This technical series explains why CLEVER — an antimicrobial peptide concentrate from yeast — combined with antibiotics produces a synergistic effect: stronger pathogen kill, lower antibiotic dose, less resistance gene selection pressure, and protected gut mucosa.

View original brochure ↗Zalo support 0867 957 568

1. Why combine CLEVER with antibiotics?

CLEVER and antibiotics act on three parallel fronts: complementary entry mechanism, biofilm disruption, and gut microbiome balance. On their own, antibiotics struggle to reach bacteria hiding in biofilms; peptides only suppress at the population level. Together, the mechanisms cover each other's gaps — bacterial kill rises sharply while individual doses drop.

Complementary mechanism: peptides create membrane pores → antibiotics penetrate more easily → lower dose required.
Biofilm synergy: peptides break biofilms → antibiotics reach the hidden bacteria → reduced inflammation, faster tissue recovery.
Gut effect: CLEVER kills Vibrio while activating the immune response — shrimp stay healthier without losing beneficial gut microbes.

Three synergy mechanisms between CLEVER and antibiotics — page 1.

2. Scientific basis — antimicrobial peptides + antibiotics

Studies on E. coli show that LL-37 peptide combined with colistin drops bacterial counts below detection while colistin dose can be cut to a fraction of monotherapy levels. This is the foundation for CLEVER (yeast peptide concentrate) plus antibiotics — lower antibiotic dose, equal or stronger kill.

Membrane permeability: peptides disrupt the bacterial membrane and create pores → antibiotic uptake jumps multi-fold.
Biofilm disruption: only when the biofilm breaks does the antibiotic actually work — critical for gut Vibrio.
Immune modulation: CLEVER activates TLR/NF-κB → balanced cytokines → less inflammation, faster mucosal regeneration → faster shrimp recovery.

Three-layer mechanism: permeability, biofilm, immune modulation (page 2).

3. Benefits of CLEVER + antibiotic combinations

Farmers applying this combo correctly see 5 core benefits — affecting both the current cycle and the long-term health of pond environments:

Stronger antibacterial effect than antibiotic monotherapy.
Lower antibiotic dose — reduces selection pressure for resistance genes in the pond environment.
Less resistance: high-dose antibiotics are the main driver of resistance gene emergence — pairing with CLEVER cuts that loop.
Healthier gut: beneficial microbiome diversity preserved, gut mucosa intact, hepatopancreas spared.
Long-term safety: lower residues, stable environment, easier next cycle.

Five benefits of CLEVER + antibiotic combinations (page 3).

4. Usage guide — prevention and treatment

Exact dosing depends on pond status, shrimp age, and disease type. Here is Than Vuong's standard combination framework:

Disease prevention: mix CLEVER 5 – 10 g/kg feed + low-dose antibiotic (e.g., TETRA MAX) → control Vibrio spp. starting at the high-risk stage.
White feces / TPD treatment: increase CLEVER + antibiotic doses — synergy reduces total antibiotic over the full course.
Pick the right pairing: not every peptide + antibiotic combo is synergistic — call our technicians for a recommendation based on pond status and antibiogram.
Note: only use antibiotics after diagnostic confirmation (PCR, antibiogram). Use the right protocol — full dose, full course — with CLEVER throughout to protect the gut microbiome.

Prevention – treatment – combination framework (page 4).

5. Cautions & recommendations

Peptide + antibiotic combinations are a major step forward in shrimp disease protocols — but only when used correctly:

Do not replace antibiotics with CLEVER alone in acute septicemia — CLEVER is a support, not a stand-alone cure for systemic infection.
Always use antibiotics with an antibiogram — wrong-target choice accelerates resistance.
Run CLEVER continuously even in prevention to maintain immunity and reduce antibiotic frequency.
Treat pond water (Vibrio Clear, MAX 4000) in parallel to remove pathogens from the water column.

📋 Official dosage from product label

Verbatim from the CLEVER (200 g) label — synced with the product page to avoid drift.

Ingredients: Protein (extracted from yeast).

Directions & dosage:

Mix the product with a sufficient amount of clean water, mix thoroughly into the feed, coat with ATOMIC or a suitable coating product, and let dry for 20 minutes before feeding to shrimp. Routine use: 2 – 3 g/kg feed, 2 times/day. For periods requiring intestinal support: 5 – 7 g/kg feed, 4 times/day. Can be fed in combination with FLASH to enhance effectiveness. Note: Seal tightly, zip the bag immediately after use if the product is not fully consumed.

Label benefits: