📘 Official technical document

Microbiome Farm Protocol

Prevention beats treatment — by building a microbial ecosystem across the entire crop.

Version: 2026.04·For: Whiteleg shrimp (P. vannamei) + Black tiger shrimp

Contents:1. Challenges 2. Shrimp gut microbiome 2B. Microbiome diversity 3. 4 solution pillars 4. Phase-based application 4B. Customer success 5. References

Challenges shrimp farmers face

4 major diseases threaten the shrimp industry. The first 3 have single causative agents. WFS — the most common — results from MULTIPLE causes and cannot be solved with antibiotics alone.

WSD

White Spot Disease

Single agent — WSSV virus

EMS

Early Mortality Syndrome

Single agent — Vibrio parahaemolyticus / AHPND

TPD

Translucent Postlarvae Disease

Single agent — emerging Vibrio strain

WFS

White Feces Syndrome

MULTI-CAUSAL — EHP + Vibrio + parasites + dysbiosis

⚠ Multi-causal

Implication: No single drug solves WFS. We must build a healthy microbiome + control multi-layer agents BEFORE outbreak.

Shrimp gut microbiome

Modern metagenomics lets us classify shrimp gut bacteria into 3 groups: beneficial, opportunistic, pathogenic. The balance among these 3 determines shrimp health.

Beneficial

Bacillus
Lactococcus
Lactobacillus
Pediococcus
Bifidobacterium
Rhodopseudomonas

Opportunistic

Pseudomonas
Flavobacterium

Pathogenic

Vibrio parahaemolyticus
Vibrio harveyi
EHP

Reference: Cornejo-Granados et al., 2017 — Scientific Reports

02·B

Microbiome diversity — 3 layers in parallel

The Microbiome Farm Protocol does NOT rely on a single product. Diversity is built through 3 parallel layers: pond (led by SOILMAX), gut (Bio Max + feed line), and host (hepatopancreas + immunity). Each layer adds distinct microbial strains — yielding a diverse, more disease-resistant ecosystem.

POND

Pond bottom & water microbiome

SOILMAX New + LALSEA BIOREM + MAX 4000 + OZYME

Decompose organics, suppress Vibrio, remove NH3/NO2/H2S, stabilise nitrogen cycle

GUT

Shrimp gut microbiome

BIO MAX + PRO GUT + FLASH + CLEVER + ACTISEL

Postbiotic + probiotic + live yeast — displace pathogens, boost beneficial Bifidobacteria

HOST

Host (shrimp) support

HERB GUARD + AQUAMOS + CANFORTA + CELL CANPHOS GOLD

Protect hepatopancreas, boost immunity, supply trace minerals — strengthen immune system

Synthesis: The 3 layers operate in parallel, not as substitutes. SOILMAX in pond + Bio Max in gut + HERB GUARD on host → shrimp have a diverse microbiome and far higher natural resistance than farms using only one product line.

4 Than Vuong solution pillars

Each pillar tackles a distinct layer. Apply all 4 simultaneously to fulfil the complete Microbiome Farm Protocol.

POSTBIOTIC ISAPP

White Feces Syndrome Prevention

BIO MAX — Postbiotic per ISAPP standard

Bio Max contains INACTIVE microbes + metabolites (organic acids, peptides, exopolysaccharides). No live microbes required — active on contact with gut.

Mechanisms

Protect tight junctions — sealed gaps between gut cells
Prevent leaky gut — block pathogens & toxins from blood
Stimulate beneficial Bifidobacteria within 24h
Anti-inflammatory + post-antibiotic recovery

📜 Salminen et al. (ISAPP) (2021) — DOI: 10.1038/s41575-021-00440-6

REBALANCING GUT

Gut Rebalance + Hepatopancreas Care

PRO SUR — Lipids from palm & coconut oil

Hepatopancreas handles 5 functions: energy reserve, digestion, immunity, detox (mycotoxin/endotoxin), hematopoiesis. Pro Sur supplies lipids → complete moulting, no stuck shells.

Mechanisms

Boost fat utilization, reduce liver fat accumulation
Support weight gain on high-fat diets
Improve digestion & nutrient absorption
Strengthen innate immunity

📜 Cornejo-Granados et al. (2017) — DOI: 10.1038/s41598-017-11805-w

LIQUID TRACE MINERALS

Liquid Trace Minerals — Soft Shell

CELL CANPHOS GOLD (water apply) + CALPHOS GRO (feed mix)

CELL CANPHOS GOLD liquid form applied to pond — pre-ionised Ca²⁺/Mg²⁺, PASSIVELY absorbed via gills in 2–4h. Combined with CALPHOS GRO 3–5 ml/kg feed for gut mineral. Chelated organic minerals absorbed better than typical inorganic minerals.

Mechanisms

CELL CANPHOS GOLD 1kg/1,000m³ in water — liquid mineral, gill absorption in 2–4h
CALPHOS GRO 3–5 ml/kg feed — gut mineral + nutrient transport
Nutrition balance (Ca, Mg, P, Zn, Cu, Se) across moulting cycles
Resolve soft shell syndrome — ref. Prof. Orapint Jintasatporn

📜 Aranguren et al. (2021) — DOI: 10.1016/j.aquaculture.2020.736035

GROWTH SUPPORT

Protein/energy ratio + Growth support

Combination — CANFORTA + ACTISEL + AQUAMOS

Optimise protein/energy ratio in feed. Full trace minerals + enhanced absorption + rapid shell hardening. This completes the Microbiome Farm cycle.

Mechanisms

Optimise protein/energy (P/E) ratio
Full trace minerals at every stage
Enhanced mineral absorption (CELL CANPHOS × ACTISEL synergy)
Rapid shell hardening — even and strong sizing

Phase-based application

Each phase has a specific microbial objective. Right product — right phase — right dose.

Phase	DOC	Key products	Goal
Pond prep	`Day -7 → 0`	SOILMAX New + MAX 4000 + OZYME	Build microbiome foundation pre-stocking
Early grow-out	`Day 1 → 30`	PRO GUT + FLASH + CLEVER + BIO MAX	Gut microbiome — prevent WFS at root
Mid grow-out⭐	`Day 30 → 60`	LALSEA BIOREM + SOILMAX + HERB GUARD	Stabilise water + bottom — control Vibrio
Finishing	`Day 60 → harvest`	CELL CANPHOS GOLD + CALPHOS GRO + CANFORTA + AQUAMOS	Hepatopancreas + shell + size

See Microbiome Farm Protocol Timeline →

04·B

Customer success with Microbiome Farm Protocol

3 partner farms applied the full Microbiome Farm Protocol — not a campaign, just real crops.