POND
Pond bottom & water microbiome
SOILMAX New + LALSEA BIOREM + MAX 4000 + OZYME
Decompose organics, suppress Vibrio, remove NH3/NO2/H2S, stabilise nitrogen cycle
📘 Official technical document
Microbiome Farm Protocol
Prevention beats treatment — by building a microbial ecosystem across the entire crop.
01
Challenges shrimp farmers face
4 major diseases threaten the shrimp industry. The first 3 have single causative agents. WFS — the most common — results from MULTIPLE causes and cannot be solved with antibiotics alone.
WSD
White Spot Disease
Single agent — WSSV virus
EMS
Early Mortality Syndrome
Single agent — Vibrio parahaemolyticus / AHPND
TPD
Translucent Postlarvae Disease
Single agent — emerging Vibrio strain
WFS
White Feces Syndrome
MULTI-CAUSAL — EHP + Vibrio + parasites + dysbiosis
⚠ Multi-causalImplication: No single drug solves WFS. We must build a healthy microbiome + control multi-layer agents BEFORE outbreak.
02
Shrimp gut microbiome
Modern metagenomics lets us classify shrimp gut bacteria into 3 groups: beneficial, opportunistic, pathogenic. The balance among these 3 determines shrimp health.
Beneficial
- Bacillus
- Lactococcus
- Lactobacillus
- Pediococcus
- Bifidobacterium
- Rhodopseudomonas
Opportunistic
- Pseudomonas
- Flavobacterium
Pathogenic
- Vibrio parahaemolyticus
- Vibrio harveyi
- EHP
Reference: Cornejo-Granados et al., 2017 — Scientific Reports
02·B
Microbiome diversity — 3 layers in parallel
The Microbiome Farm Protocol does NOT rely on a single product. Diversity is built through 3 parallel layers: pond (led by SOILMAX), gut (Bio Max + feed line), and host (hepatopancreas + immunity). Each layer adds distinct microbial strains — yielding a diverse, more disease-resistant ecosystem.
GUT
Shrimp gut microbiome
BIO MAX + PRO GUT + FLASH + CLEVER + ACTISEL
Postbiotic + probiotic + live yeast — displace pathogens, boost beneficial Bifidobacteria
HOST
Host (shrimp) support
HERB GUARD + AQUAMOS + CANFORTA + CELL CANPHOS GOLD
Protect hepatopancreas, boost immunity, supply trace minerals — strengthen immune system
Synthesis: The 3 layers operate in parallel, not as substitutes. SOILMAX in pond + Bio Max in gut + HERB GUARD on host → shrimp have a diverse microbiome and far higher natural resistance than farms using only one product line.
03
4 Than Vuong solution pillars
Each pillar tackles a distinct layer. Apply all 4 simultaneously to fulfil the complete Microbiome Farm Protocol.
01
POSTBIOTIC ISAPPWhite Feces Syndrome Prevention
BIO MAX — Postbiotic per ISAPP standard
Bio Max contains INACTIVE microbes + metabolites. No live microbes required — active on contact with gut.
Mechanisms
📜 Salminen et al. (ISAPP) (2021) — DOI: 10.1038/s41575-021-00440-6- Protect tight junctions
- Prevent leaky gut
- Stimulate beneficial Bifidobacteria within 24h
- Anti-inflammatory + post-antibiotic recovery
02
REBALANCING GUTGut Rebalance + Hepatopancreas Care
PRO SUR — Lipids from palm & coconut oil
Hepatopancreas handles 5 functions. Pro Sur supplies lipids → complete moulting, no stuck shells.
Mechanisms
📜 Cornejo-Granados et al. (2017) — DOI: 10.1038/s41598-017-11805-w- Boost fat utilization
- Support weight gain on high-fat diets
- Improve digestion & nutrient absorption
- Strengthen innate immunity
03
TRACE MINERALSTrace Minerals — Soft Shell
CELL CANPHOS GOLD + CALPHOS GRO
CELL CANPHOS GOLD dissolves in water → Ca²⁺/Mg²⁺ ions absorbed via gills. CALPHOS GRO feed mix delivers gut minerals.
Mechanisms
📜 Aranguren et al. (2021) — DOI: 10.1016/j.aquaculture.2020.736035- Gill absorption in 2–4h
- 3–5 ml/kg feed — gut mineral
- Nutrition balance (Ca, Mg, P, Zn, Cu, Se)
- Resolve soft shell syndrome
04
GROWTH SUPPORTProtein/energy ratio + Growth support
CANFORTA + ACTISEL + AQUAMOS
Optimise protein/energy ratio. Full trace minerals + enhanced absorption + rapid shell hardening.
Mechanisms
- Optimise P/E ratio
- Full trace minerals
- Enhanced mineral absorption
- Rapid shell hardening
04
Phase-based application
Each phase has a specific microbial objective. Right product — right phase — right dose.
| Phase | DOC | Key products | Goal |
|---|---|---|---|
| Pond prep | Day -7 → 0 | SOILMAX New + MAX 4000 + OZYME | Build microbiome foundation pre-stocking |
| Early grow-out | Day 1 → 30 | PRO GUT + FLASH + CLEVER + BIO MAX | Gut microbiome — prevent WFS at root |
| Mid grow-out⭐ | Day 30 → 60 | LALSEA BIOREM + SOILMAX + HERB GUARD | Stabilise water + bottom — control Vibrio |
| Finishing | Day 60 → harvest | CELL CANPHOS GOLD + CALPHOS GRO + CANFORTA + AQUAMOS | Hepatopancreas + shell + size |
04·B
Customer success with Microbiome Farm Protocol
3 partner farms applied the full Microbiome Farm Protocol — not a campaign, just real crops.
Microbiome Farm Protocol — first applied crop
Mr. Nguyễn Văn A's farm — Sóc Trăng
📊 92% survival
WFS controlled from day 15
Mr. Trần Văn B's farm — Cà Mau
📊 FCR 1.05
High-density lined pond — even size
Mr. Lê Văn C's farm — Bến Tre
📊 +250M VND/crop
Have you applied the Microbiome Farm Protocol and want to share your crop? Submit your story via Zalo →
05
References
- Cornejo-Granados et al. (2017) — Whiteleg shrimp gut microbiome — metagenomics analysis. Scientific Reports. DOI: 10.1038/s41598-017-11805-w
- Aranguren et al. (2021) — Etiology of WFS in Penaeus vannamei. Aquaculture. DOI: 10.1016/j.aquaculture.2020.736035
- Salminen et al. — ISAPP (2021) — ISAPP consensus definition of Postbiotic. Nature Reviews Gastroenterology & Hepatology. DOI: 10.1038/s41575-021-00440-6
- Hill et al. — ISAPP (2014) — ISAPP consensus definition of Probiotic. Nature Reviews Gastroenterology & Hepatology. DOI: 10.1038/nrgastro.2014.66
- Gibson et al. — ISAPP (2017) — ISAPP consensus definition of Prebiotic. Nature Reviews Gastroenterology & Hepatology. DOI: 10.1038/nrgastro.2017.75
- Prof. Orapint Jintasatporn — Personal reference on soft shell syndrome in shrimp. Kasetsart University, Thailand
- Zou et al. (2020) — TPD — emerging Vibrio pathogen. Aquaculture. DOI: 10.1016/j.aquaculture.2020.735581
Apply Microbiome Farm Protocol to your pond
Contact Than Vuong's technical team for a custom protocol based on your pond area, density, and stage.